@TOME V2.2
(Nov 2016)
Ref.
-
Doc.
Global output mode :
Sort entries by :
Atome Result
Tools Result
PDB occurence
Core Structure Cluster
Sequence Color type :
Secondary Structure
Hydrophobicity
Disulfide Bonds
TCoffee Identity Score
Uniprot annotation
Core Structure Cluster
Contacts with Ligands
Show alignment :
Atome selection
All alignments
Only Template with Ligand
Column output:
Score:
Atome Rank Value
Tools Score
Hide column
Alignment:
Sequence Identity (%)
Tcoffee Alignment Score
Hide column
3D Common Core:
Tito PKB Score
Verify3D Score
QMean Score
Hide column
Structural Clustering:
Cluster Num
3D-Jury Struct. Score
Hide column
Modeller Result :
Model Rank
QMean Score
Dfire Score
Dope Score
Errat Score
Verify3D Score
Modeller Score
Hide column
Complexes Modeling
Show Ligands of Templates
Complexes Affinity Score
Main Binding Site Identity
Hide column
Templates Information:
Uniprot entry
Cath class
Scop class
Smart class
Prosite class
Pfam class
EC Number
Taxonomy
Experiment Method
Hide column
Sequence & Result Tab:
PDB Template Sequence
Complexes Tab
Values color: [
Good
|
Correct
|
Middling
|
Bad
]
Sequence color :
Mutated
Helix
Strand
Conserved
Query sequence : S20A1_HUMAN: (2015-12-09 )
MATLITSTTAATAASGPLVDYLWMLILGFIIAFVLAFSVGANDVANSFGTAVGSGVVTLKQACILASIFETVGSVLLGAKVSETIRKGLIDVEMYNSTQGLLMAGSVSAMFGSAVWQLVASFLKLPISGTHCIVGATIGFSLVAKGQEGVKWSELIKIVMSWFVSPLLSGIMSGILFFLVRAFILHKADPVPNGLRALPVFYACTVGINLFSIMYTGAPLLGFDKLPLWGTILISVGCAVFCALIVWFFVCPRMKRKIEREIKCSPSESPLMEKKNSLKEDHEETKLSVGDIENKHPVSEVGPATVPLQAVVEERTVSFKLGDLEEAPERERLPSVDLKEETSIDSTVNGAVQLPNGNLVQFSQAVSNQINSSGHYQYHTVHKDSGLYKELLHKLHLAKVGDCMGDSGDKPLRRNNSYTSYTMAICGMPLDSFRAKEGEQKGEEMEKLTWPNADSKKRIRMDSYTSYCNAVSDLHSASEIDMSVKAEMGLGDRKGSNGSLEEWYDQDKPEVSLLFQFLQILTACFGSFAHGGNDVSNAIGPLVALYLVYDTGDVSSKVATPIWLLLYGGVGICVGLWVWGRRVIQTMGKDLTPITPSSGFSIELASALTVVIASNIGLPISTTHCKVGSVVSVGWLRSKKAVDWRLFRNIFMAWFVTVPISGVISAAIMAIFRYVILRM
Atome Classification :
(20 SA)
--------.........10........20........30........40........50........60........70.......--.80........90........100.......110.......120.......130.......140.......150..-.....160.......170.......180.......190.......200.......210.......220.......230.......240.......-250-...--------....260..-.....270..-----.....280.......--290---.....-.--.300.......310.....---.-.320.......330.......340.......350.......360.......370.......380.......390.......400...--....410.......420.......430.......440.......450.......460.......470.......480.......490.......500.......510.......520.......530.......540...----....550.......560.......570.......580.......590.....-..600.......610.......620.......630.--......640.......--650.......660.......670.......
(Atome)
(Ident)
(Tito)
(Num)
(Ligand)
(Uniprot)
--------M
A
T
L
I
TSTTAATAASG
P
L
V
D
Y
L
W
M
L
I
L
G
F
I
I
A
F
V
L
A
F
SVG
A
N
D
V
A
N
S
F
G
T
A
VG
S
G
V
V
T
L
K
Q
A
C
I
L
A
S
I
F
E
T
V
G
S
V
L
LG--
A
K
V
S
E
T
I
R
KG
L
I
D
V
E
M
YNSTQ
G
L
L
M
A
G
S
V
S
A
M
F
G
S
A
V
W
Q
L
V
A
S
F
L
KLPISGT
H
C
I
V
GATI
G
F
S
L
VAKGQEGV
K
W
S
E
-
L
I
K
I
V
M
S
W
F
V
S
P
L
L
S
G
I
M
S
G
I
L
F
F
L
V
R
A
F
I
LHKADPVPNG
L
R
A
L
P
V
F
Y
A
C
T
V
G
I
N
L
F
S
I
M
YTGAPLLGFDKLP
L
W
G
T
I
L
I
S
V
G
C
A
V
F
C
A
L
I
V
W
F
F
-V
C
P
-
R
M
K
--------
R
K
I
E
R
E
IKC-SPSES
P
L
M
E
K
-----
K
N
S
L
K
E
D
H
E
E
T
K
L
SV--GDI---ENKHP-V--SEVGPAT
V
P
L
Q
A
V
V
E
ER
T
V
---
S
-
F
KLG
D
L
EEAP
E
R
ERLPSVDLKEETSIDSTVNG
A
V
QLPNG
N
L
V
Q
F
S
Q
A
V
S
NQINSSG
H
Y
Q
Y
H
T
VHKDS
G
L
Y
K
E
L
L
H
K
L
H
L
A
K
V
G
D
CMG--DSGDKPLRRNNSY
T
S
Y
T
M
A
I
CGMP
L
D
S
F
RAKEGE
Q
K
G
E
E
M
E
KLTWPNADSK
K
R
I
RMD
S
Y
T
S
Y
C
N
A
V
S
D
LH
S
A
S
EI
D
M
S
V
K
A
EMGLGDRKGSNG
S
L
E
E
W
Y
DQDKP
E
V
S
L
L
F
Q
F
L
Q
I
L
T
A
C
F
G
S
FAHGGN
D
V
S
N
A
I
G
P
L
V
A
L
----
Y
L
V
Y
DTGDVSSKVAT
P
I
W
L
L
L
YG
G
V
G
I
C
V
G
L
W
V
W
G
R
R
V
I
Q
T
MGKDLTPITPS-SG
F
S
I
E
L
A
S
A
L
T
V
V
I
ASNIGLPIST
T
H
C
KVG
S
V
V
S
V
--
G
WLRSKKA
V
D
W
R
L
F
R
N
--
I
F
M
A
W
F
V
TV
P
I
S
G
V
I
S
A
A
I
M
A
I
F
R
Y
V
I
LRM
22
Fugue
94.09
16%
-65
- C4 -
4NFU
3
EDS1L_ARATH
A:
[15-633]
AFEALTGI
N
G
D
L
I
T
R
S
W
S
A
S
K
Q
A
Y
L
T
E
R
Y
H
K
E
E
A
-
G
A
V
V
I
F
A
F
Q
P
S
F
S
E
K
D
F
F
D
P
-
D
N
K
S
S
F
G
E
I
K
L
N
R
V
-----
Q
F
P
C
M
R
K
I
G
K
G
D
V
A
T
V
N
E
A
F
L
K
N
L
E
A
V
I
------------
D
P
R
T
S
F
Q
A
S
V
E
M
A
V
R
S
R
K
Q
I
V
F
T
G
H
S
-----------------------------------------
S
G
G
A
T
A
I
L
A
T
V
W
Y
L
E
K
Y
F
I
R
N
P
N
V
Y
L
E
P
R
C
V
T
F
------------
G
A
P
L
V
G
D
---
S
I
F
S
H
A
L
G
R
E
K
W
S
R
F
F
V
N
F
V
T
R
F
D
I
V
P
-
R
I
T
LARKASVE
E
T
L
P
H
V
L
A
Q
L
D
P
R
N
S
S
V
Q
E
S
-----
E
Q
R
I
T
E
F
Y
T
S
V
M
R
D
T
--
S
T
V
---
A
N
Q
A
V
-
C
--
E
L
T
G
S
A
E
A
I
L
E
T
L
S
S
F
L
E
L
---
S
P
Y
R
P
A
G
T
F
V
F
S
T
E
K
R
L
V
A
V
N
--
N
S
D
A
I
L
Q
M
L
F
Y
T
C
Q
A
S
D
E
Q
--
E
W
S
L
I
P
F
R
S
I
R
D
H
H
S
Y
E
E
L
V
Q
S
M
G
M
K
L
F
N
H
L
D
G
E
N
S
I
E
S
S
L
N
D
L
G
VS
T
R
G
R
Q
Y
V
Q
A
A
L
E
E
E
K
K
R
V
E
N
Q
K
K
I
I
Q
V
I
Q
Q
E
R
F
L
K
K
L
A
W
I
E
D
E
Y
K
P
K
C
Q
A
H
K
N
G
Y
Y
D
S
F
K
V
S
N
E
E
N
D
F
K
A
N
V
K
R
A
E
L
A
G
V
F
D
E
V
L
G
L
L
K
--
K
C
Q
L
P
D
E
F
E
G
D
I
D
W
I
K
L
A
T
R
Y
R
R
L
V
E
P
L
D
I
A
N
Y
H
R
H
L
K
N
E
D
T
-
G
P
Y
M
K
R
GRP
T
R
Y
I
Y
A
Q
R
G
Y
E
H
H
I
L
K
P
N
--------
G
M
I
A
E
D
V
F
W
N
K
V
N
G
L
N
L
G
L
Q
L
E
E
I
Q
E
T
L
K
N
S
G
S
E
C
G
S
C
F
W
A
E
V
E
E
L
K
G
K
P
Y
E
E
V
E
V
R
V
K
T
L
E
G
M
L
R
E
W
I
T
A
G
E
V
D
E
K
E
I
F
L
E
GS
T
F
R
K
W
W
I
T
L
P
K
N
H
K
S
H
S
P
L
R
D
Y
M
M
D
E
I
---
14
Fugue
49.58
13%
79
- C6 -
4GKW
-
SAS6_CAEEL
A:
[250-407]
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
E
D
K
E
D
E
V
A
D
L
K
Q
D
T
---
E
-
S
L
Q
K
Q
L
E
E
N
Q
E
E
L
E
I
V
G
N
M
L
R
E
E
Q
G
K
V
D
Q
L
Q
K
R
N
V
A
H
Q
K
E
I
G
K
L
R
A
E
L
G
T
A
Q
R
N
L
E
K
A
D
Q
L
L
K
R
N
S
Q
Q
Q
N
Q
Q
S
L
D
M
R
K
L
G
E
L
E
A
-------
D
L
K
E
K
D
S
M
V
E
S
L
T
E
T
I
G
I
L
R
K
E
L
E
N
E
K
L
K
A
A
E
N
M
-
D
S
F
E
K
L
S
M
E
N
E
N
L
K
E
K
I
A
H
Y
R
A
Q
R
F
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
2
PsiBlast_PDB
49.57
31%
116
- C5 -
3PG7
-
NF1_HUMAN
A:
[128-214]
------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
F
I
-
D
C
P
G
K
L
A
--------
E
H
I
E
H
E
Q
Q
K
-
L
P
A
A
T
L
A
L
E
E
DLKVF
H
N
A
L
K
L
A
H
K
D
T
K
V
S
I
------------------
K
V
G
S
T
A
V
--
Q
V
T
S
A
E
R
T
V
LG
Q
S
-
V
F
L
N
D
I
Y
Y
A
S
E
I
E
E
I
C
L
V
D
E
N
Q
F
T
L
T
I
A
N
Q
G
T
P
L
T
F
M
H
Q
E
C
E
A
I
V
Q
S
I
I
H
I
R
T
R
W
E
L
S
Q
P
D
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
3
PsiBlast_PDB
46.88
32%
119
- C5 -
3A06
2
DXR_THEMA
A:
[186-245]
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
K
H
P
-
V
--
W
N
M
G
A
-----
R
I
T
V
D
S
A
T
M
---
V
-
N
K
A
F
E
V
L
E
A
M
E
L
F
E
L
P
F
E
K
I
E
V
K
I
H
R
E
G
L
V
H
G
A
V
V
L
P
D
G
N
V
K
M
V
V
S
P
P
D
M
R
I
P
I
S
Y
A
L
F
Y
P
R
R
V
A
L
E
P
F
F
L
R
T
I
S
L
S
F
E
D
P
D
P
E
K
Y
P
--
A
F
F
L
L
K
E
I
K
D
S
Y
A
L
R
T
A
F
N
A
A
D
E
V
A
V
E
A
F
L
K
G
R
I
R
F
G
G
I
H
R
V
I
E
K
T
L
E
E
F
Q
G
Y
P
Q
P
R
T
L
D
D
V
E
R
I
H
F
E
A
I
K
K
A
E
R
V
T
E
W
L
S
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
17
Fugue
46.71
8%
162
- C6 -
4H22
-
LRRF1_HUMAN
A:
[168-248]
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
E
E
-
K
Y
K
--------
K
A
M
V
S
N
A
Q
L
-
D
N
E
K
T
N
F
M
Y
Q
-----
V
D
T
L
K
D
M
L
L
E
L
E
E
Q
L
--
A
E
S
---
R
R
Q
Y
E
-
E
--
K
N
K
E
F
E
R
E
K
H
A
H
S
I
L
Q
F
Q
F
---
A
-
E
V
K
E
A
L
K
Q
R
E
E
M
L
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
15
Fugue
46.71
16%
174
- C6 -
1X8Y
-
LMNA_HUMAN
A:
[313-386]
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
L
-
A
A
K
E
A
K
L
R
D
L
-----
E
D
S
L
A
R
E
R
D
T
S
R
R
L
L
--
A
E
K
---
E
R
E
M
A
-
E
--
M
R
-
A
R
M
Q
Q
Q
L
D
E
Y
Q
E
L
L
D
I
---
K
-
L
A
L
-
D
M
E
I
H
A
Y
R
K
L
L
E
G
E
E
E
R
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
18
Fugue
45.38
7%
99
- C6 -
4PXU
1
KL61_DROME
A:
[8-159]
------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
M
S
K
E
I
Q
T
N
-----
L
Q
V
I
E
E
N
N
Q
R
H
K
A
M
L
--
D
S
M
---
Q
E
K
F
A
T
I
--
I
D
S
S
L
Q
S
V
E
E
H
A
K
Q
M
H
K
K
L
---
E
-
Q
L
G
A
M
S
L
P
D
A
E
E
L
Q
N
L
Q
E
E
L
A
N
E
R
A
L
A
Q
Q
E
D
A
L
L
E
S
M
M
M
Q
M
E
Q
I
K
N
L
R
S
K
N
S
I
S
M
S
V
H
L
N
K
M
E
E
S
R
L
T
R
N
H
R
I
D
D
I
K
S
G
I
Q
D
Y
Q
K
L
G
--
I
E
A
S
Q
S
A
Q
A
E
L
T
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
19
Fugue
44.53
10%
105
- C6 -
2B9C
-
TPM1_RAT
A:
[98-233]
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
E
L
D
R
A
-
Q
E
R
L
A
T
A
L
Q
K
-----
L
E
E
A
E
K
A
A
D
E
S
E
R
G
M
--
K
V
I
---
E
S
R
A
Q
-
K
--
D
E
E
K
M
E
I
Q
E
I
Q
L
K
E
A
K
H
I
A
---
E
-
D
A
D
R
K
Y
E
E
V
A
R
K
L
V
I
I
E
S
D
L
E
R
A
E
E
R
A
E
L
S
E
G
K
C
A
E
L
E
E
E
L
K
T
V
T
N
N
L
K
S
L
E
D
K
V
E
E
L
L
S
K
N
Y
H
L
E
N
E
V
-
A
R
L
K
K
L
V
G
------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
23
Fugue
40.77
10%
125
* C6 *
1L8D
1
?
A:
[2-104]
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
K
K
-
L
L
E
--------
E
L
E
T
K
K
T
T
I
-
E
E
E
R
N
E
I
T
Q
R
-----
I
G
E
L
K
N
K
I
G
D
L
K
T
A
I
--
E
E
L
K
K
A
K
G
K
C
P
-
V
CG
R
E
L
T
D
E
H
R
E
E
L
L
S
K
Y
H
L
D
L
---
N
-
N
S
K
N
T
L
A
K
L
I
D
R
K
S
E
L
E
R
E
L
R
R
I
D
M
E
I
K
R
L
------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
21
Fugue
39.54
29%
559
- C6 -
2DOH
-
C:
[4-28]
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
L
T
A
D
A
E
L
Q
R
L
-----
K
N
E
R
H
E
E
A
E
L
E
R
L
S
------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
20
Fugue
38.45
13%
141
- C6 -
4YVQ
-
A:
[1-89]
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
M
L
E
T
-
V
P
T
I
K
K
L
R
A
Y
-----
A
E
R
I
R
V
A
E
L
E
K
C
M
S
K
M
G
D
D
I
---
N
K
K
T
T
-
R
--
A
V
D
D
L
S
R
G
I
V
N
R
F
L
H
G
P
M
Q
---
H
-
L
R
C
R
T
L
S
E
T
L
E
N
M
H
A
L
N
R
M
Y
G
L
E
K
------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
1
PsiBlast_PDB
37.30
34%
107
- C5 -
3S9X
-
?
A:
[27-83]
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
E
K
Y
T
S
F
S
---
A
D
Y
F
C
A
D
E
Y
N
A
N
V
C
A
D
L
I
L
R
G
E
K
R
A
S
C
S
L
E
Y
W
Y
S
Q
-
K
G
E
L
M
P
Q
V
G
H
L
Q
V
V
T
N
W
D
G
K
P
I
C
I
I
E
I
T
S
V
S
K
C
Q
Y
N
Q
V
----
S
E
D
F
A
A
S
E
G
E
G
D
K
S
L
A
W
W
Q
E
A
H
R
N
F
F
S
R
E
C
H
E
L
G
I
E
F
R
E
D
M
L
L
V
L
E
H
F
K
V
V
Y
-
H
-------------------------------------------------------------------------------------
16
Fugue
37.11
26%
520
- C5 -
2LMF
-
A:
[1-23]
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
L
L
G
D
F
F
R
K
S
K
E
K
I
G
K
E
F
K
R
I
V
Q
R
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
10
HHSearch
36.01
15%
265
- C5 -
3W9I
-
MEXB_PSEAE
A:
[1005-1030]
------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
V
I
G
G
M
V
T
A
T
V
L
A
I
F
-
W
V
P
-
L
F
Y
--------
V
A
V
S
T
L
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
13
HHSearch
26.44
15%
257
* C5 *
2KSD
-
ARCB_ECOLI
A:
[49-75]
--------------------------------------------------------------------------------------------------------------------------------------------------------------
Q
V
E
S
I
D
V
I
R
S
I
F
F
G
L
L
I
T
P
W
A
V
Y
F
L
S
V
V
V
E
Q
L
E
E
S
R
Q
R
L
S
R
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
6
HHSearch
23.62
57%
1083
* C7 *
2FSV
1
C:
[125-138]
-------------------------------------------
A
F
V
I
G
A
N
D
V
T
N
P
A
A
------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
8
HHSearch
22.63
57%
924
- C7 -
1PNO
1
PNTB_RHORT
A:
[102-115]
-------------------------------------------
A
F
V
I
G
A
N
D
V
T
N
P
A
A
K
T
D
P
S
S
P
I
Y
G
M
P
I
L
D
V
E
K
A
G
T
V
L
F
I
K
R
S
M
--
A
S
G
Y
A
G
V
E
N
E
L
F
F
R
N
N
T
M
M
L
F
G
D
A
K
K
M
T
E
Q
I
V
Q
A
M
N
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
12
HHSearch
21.13
16%
521
- C5 -
2LS4
-
A:
[4-22]
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
Y
L
C
I
A
V
A
A
G
A
G
T
G
Y
F
L
F
S
W
K
K
-----------------------------------------------------------------------------------------------------
9
HHSearch
20.46
33%
400
- C5 -
3WU2
-
PSBH_THEVL
H:
[15-38]
------------------------------------------------------------------------------------------------------------------------------------------------------------------
S
E
-
Y
G
K
V
A
P
G
W
G
T
T
P
L
M
A
V
F
M
G
L
F
L
V
F
L
L
I
I
L
E
I
Y
N
S
T
L
I
L
D
G
V
N
V
S
W
K
A
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
7
HHSearch
18.56
31%
860
- C7 -
2BRU
1
C:
[108-123]
------------------------------------------
T
V
L
V
I
G
A
N
D
T
V
N
P
A
A
Q
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------