Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MADKQISLPAKLINGGIAGLIGVTCVFPIDLAKTRLQNQQNG------QRVYTSMSDCLIKTVRSEGYFGMYRGAAVNLTLVTPEKAIKLAANDFFRHQL---SKDGQKLTLLKEMLAGCGAGTCQVIVTTPMEMLKIQLQDAGRIAAQRKILAAQGQLSAQGGAQPSVEAPAAPRPTATQLTRDLLRSRGIAGLYKGLGATLLRDVPFSVVYFPLFANLNQLGRPASEEKSPFYVSFLAGCVAGSAAAVAVNPCDVVKTRLQSLQRGVNEDTYSGILDCARKILRHEGPSAFLKGAYCRALVIAPLFGIAQVVYFLGIAESLLGLLQDPQA 4C9J Chain:A ((25-302)) -----TNFAINFLMGGVSAAIAKTAASPIERVKILIQNQDEMIKQGTLDKKYSGIVDCFKRTAKQEGLISFWRGNTANVIRYFPTQALNFAFKDKIKLMFGFKKEEGYGKWFAGNLASGGAAGALSLLFVYSLDF-------------ARTRLAADAKS-----ARQF--------NGLTDVYKKTLKSDGIAGLYRGFMPSVVGIVVYRGLYFGMFDSLK----------------FLLGWVVTTGASTCSYPLDTVRRRM--MMTSGQAVKYNGAIDCLKKIVASEGVGSLFKG--CGANILRSVAGAGVISMYDQLQMILFGKKF----

General information: TITO was launched using: RESULT: Template: 4C9J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -64373 for 1854 contacts (-34.7/contact) + 2D Compatibility (PS) -26129 + (NN) -3282 + (LL) 3928 1D Compatibility (HY) -14800 + (ID) 3450 Total energy: -108106.0 ( -58.31 by residue) QMean score : 0.224

(partial model without unconserved sides chains): PDB file : Tito_4C9J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4C9J-query.scw PDB file : Tito_Scwrl_4C9J.pdb: