Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTHQDLSITAKLINGGVAGLVGVTCVFPIDLAKTRLQNQHGK---------AMYKGMIDCLMKTARAEGFFGMYRGAAVNLTLVTPEKAIKLAANDFFRRLLMEDGMQRNLKMEMLAGCGAGMCQVVVTCPMEMLKIQLQDAGRLAVHHQGSASAPSTSRSYTTGSASTHRRPSATLIAWELLRTQGLAGLYRGLGATLLRDIPFSIIYFPLFANLNNLGFNELAGKASFAHSFVSGCVAGSIAAVAVTPLDVLKTRIQTLKKGLGEDMYSGITDCARKLWIQEGPSAFMKGAGCRALVIAPLFGIAQGVYFIGIGERILKCFD 2LCK Chain:A ((14-298)) --------TVKFLGAGTAACIADLITFPLDTAKVRLQIQGESQGLVRTAASAQYRGVLGTILTMVRTEGPRSLYNGLVAGLQRQMSFASVRIGLYDSVKQFYTKGSEHAGIGSRLLAGSTTGALAVAVAQPTDVVKVRFQAQARAGG---------------------GRRYQSTVEAYKTIAREEGIRGLWKGTSPNVARNAIVNCAELVTYDLIKDTLLKANLMTDDLPCHFTSAFGAGFCTTVIASPVDVVKTRYMNSAL----GQYHSAGHCALTMLRKEGPRAFYKGFMPSFLRLGSWNVVMFVTYEQLKRALMAAYQS

General information: TITO was launched using: RESULT: Template: 2LCK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -62761 for 1565 contacts (-40.1/contact) + 2D Compatibility (PS) -28226 + (NN) 1688 + (LL) 944 1D Compatibility (HY) -5600 + (ID) 3200 Total energy: -97155.0 ( -62.08 by residue) QMean score : 0.011

(partial model without unconserved sides chains): PDB file : Tito_2LCK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2LCK-query.scw PDB file : Tito_Scwrl_2LCK.pdb: