Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTAPWVALALLWGSLCAGSGRGEAETRECIYYNANWELERTNQSGLERCEGEQDKRLHCYASWRNSSGTIELVKKGCWLDDFNCYDRQECVATEENPQVYFCCCEGNFCNERFTHLPEAGGPEVTYEPPPTAPTLLTVLAYSLLPIGGLSLIVLLAFWMYRHRKPPYGHVDIHEDPGPPPPSPLVGLKPLQLLEIKARGRFGCVWKAQLMNDFVAVKIFPLQ---DKQSWQSEREIFSTPGMKHENLLQFIAAEKRGSNLEVELWLITAFHDKGSLTDYLK---GNI-ITWNELCHVAETMSRGLSYLHEDVPWCRGEGHKPSIAHRDFKSKNVLLKSDLTAVLADFGLAVRFEPGKPPGDTHGQVGTRRYMAPEVLEGAINFQRDAFLRIDMYAMGLVLWELVSRCKAADGPVDEYMLPFEEEIGQHPSLEELQEVVVHKKMRPTIKDHWLKHPGLAQLCVTIEECWDHDAEARLSAGCVEERVSLIRRSVNGTTSDCLVSLVTSVTNVDLPPKESSI 6EG9 Chain:B ((47-240)) ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GGFGVVYKGYVNNTTVAVKKLAA-ITTEELKQQFDQEIKVMAKCQHENLVELLGFSSDGD----DLCLVYVYMPNGSLLDRLSCLDGTPPLSWHMRCKIAQGAANGINFLHEN--------HH---IHRDIKSANILLDEAFTAKISDF------------------VGTTAYMAPEALRGEIT------PKSDIYSFGVVLLEIITGLPAVD------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 6EG9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 708 -16135 -22.79 -97.20 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain B : 0.61 3D Compatibility (PKB) : -22.79 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.126

(partial model without unconserved sides chains): PDB file : Tito_6EG9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6eg9-query.scw PDB file : Tito_Scwrl_6EG9.pdb: