Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL 4EOL Chain:A ((4-300)) MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRL--ETEGVPSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLSMDLKDFMDAS-LTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLR-

General information: TITO was launched using: RESULT: Template: 4EOL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1510 -178485 -118.20 -607.09 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.99 3D Compatibility (PKB) : -118.20 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.99 QMean score : 0.733

(partial model without unconserved sides chains): PDB file : Tito_4EOL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4eol-query.scw PDB file : Tito_Scwrl_4EOL.pdb: