Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGLVSSKKPDKEKPIKEKDKGQWSPLKVSAQDKDAPPLPPLVVFNHLTPPPPDEHLDEDKHFVVALYDYTAMNDRDLQMLKGEKLQVLKGTGDWWLARSLVTGREGYVPSNFVARVESLEMERWFFRSQGRKEAERQLLAPINKAGSFLIRESETNKGAFSLSVKDVTTQGELIKHYKIRCLDEGGYYISPRITFPSLQALVQHYSKKGDGLCQRLTLPCVRPAPQNPWAQDEWEIPRQSLRLVRKLGSGQFGEVWMGYYK-NNMKVAIKTLKEGTMSPE---AFLGEANVMKALQHERLVRLYAVVT-KEPIYIVTEYMARGCLLDFLKTDEGSRLSLPRLIDMSAQIAEGMAYIERMNSIHRDLRAANILVSEALCCKIADFGLARI-IDSEYTAQEGAK-FPIKWTAPEAIHFGVFTIKADVWSFGVLLMEVVTYGRVPYPGMSNPEVIRNLERGYRMPRPDTCPPELYRGVIAECWRSRPEERPTFEFLQSVLEDFYTATERQYELQP 6JMF Chain:A ((8-370)) ----------------------------------------------------------------------------------------------------------------------LHEQLWYHGAIPRAEVAELLV----HSGDFLVRESQ-GKQEYVLSV----LWDGLPRHFIIQSLDN--LYRLEGEGFPSIPLLIDHLLSTQQPLTKKSGVVLHRAVPK-----DKWVLNHEDLVLGEQIGRGNFGEVFSGRLRADNTLVAVKSCRE-TLPPDLKAKFLQEARILKQYSHPNIVRLIGVCTQKQPIYIVMELVQGGDFLTFLRT-EGARLRVKTLLQMVGDAAAGMEYLESKCCIHRDLAARNCLVTEKNVLKISDFGMSREEADGVYAASGGLRQVPVKWTAPEALNYGRYSSESDVWSFGILLWETFSLGASPYPNLSNQQTREFVEKGGRLPCPELCPDAVFR-LMEQCWAYEPGQRPSFSTIYQELQSI------------

General information: TITO was launched using: RESULT: Template: 6JMF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1816 -101833 -56.08 -286.05 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -56.08 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.336

(partial model without unconserved sides chains): PDB file : Tito_6JMF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6jmf-query.scw PDB file : Tito_Scwrl_6JMF.pdb: