Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MQPDMSLNVIKMKSSDFLESAELDSGGFGKVSLCFH--RTQGLMIMKTVYKGPNCIEHNEALLEEAKMMNRLRHSRVVKLLGVIIEEGK-YSLVMEYMEKGNLMHVLKAEMSTPLSVKGR----------IILEIIEGMCYLHGKGVIHKDLKPENILVDNDFHIKIADLGLASFKMWSKLNNEEHNELREVDGTAKKNGGTLYYMAPEHLNDVNAKPTEKSDVYSFAVVLWAIFAN-KEPYENAICEQQLIMCIKSGNRPDVDDITEYCPREIISLMKLCWEANPEARPTFPGIEEKFRPFYLSQLEESVEEDVKSLKKEYSNENAVVKRMQSLQLDCVAVPSSRSNSATEQPGSLHSSQGLGMGPVEESWFAPSLEHPQEENEPSLQSKLQDEANYHLYGSRMDRQTKQQPRQNVAYNREEERRRRVSHDPFAQQRPYENFQNTEGKGTAYSSAASHGNAVHQPSGLTSQPQVLYQNNGLYSSHGFGTRPLDPGTAGPRVWYRPIPSHMPSLHNIPVPETNYLGNTPTMPFSSLPPTDESIKYTIYNSTGIQIGAYNYMEIGGTSSSLLDSTNTNFKEEPAAKYQAIFDNTTSLTDKHLDPIRENLGKHWKNCARKLGFTQSQIDEIDHDYERDGLKEKVYQMLQKWVMREGIKGATVGKLAQALHQCSRIDLLSSLIYVSQN 1QCF Chain:A ((173-454)) KPQKP-WEKDAWEIPRESLKLEKKLGAGQFGEVWMATYNK--HTKVAVKTMKPGSM---SVEAFLAEANVMKTLQHDKLVKLHAVVTK--EPIYIITEFMAKGSLLDFLKS--------DEGSKQPLPKLIDFSAQIAEGMAFIEQRNYIHRDLRAANILVSASLVCKIADFGLARVIEDNEYTAREG------------AKFPIKWTAPEAINF--GSFTIKSDVWSFGILLMEIVTYGRIPYPGMSN-PEVIRALERGYRMPR---PENCPEELYNIMMRCWKNRPEERPTFEYIQSVLDDFYT--ATESQEEIP----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1QCF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1344 46532 34.62 175.59 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 34.62 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.175

(partial model without unconserved sides chains): PDB file : Tito_1QCF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1qcf-query.scw PDB file : Tito_Scwrl_1QCF.pdb: