Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSLPPSTSAVSSPVSSVDSPLSAVSSSIGSPGVPGTPSIGYGPISNSQINSSMSVSRLHAVSSSDDVKPPFGLKSVSGSGPMLSQKRMCAICGDRSSGKHYGVYSCEGCKGFFKRTVRKDLSYTCRDNKECLVDKRQRNRCQYCRYQKCLAMGMKREAVQEERQKNKERDGDYECSSSANEEMPVEKILEAETAVEHRTDLHSDATGSPNDPVTNICQAADKQLFTLVEWAKRVPHFSDVPLDDQVILLRAGWNELLIAAFSHRSISVKDEILLATGLHVPKESTHNLGVEAFFDRESSHSAEVGALFDRVLTELVCKMRDMQMDKTELGCLRAIVLFNPDAKGLTSSSEVELLREKVYASLESYCKQKYPDQQGRFAKLLLRLPALRSIGLKCLEHLFFFKLIGNTPIDTFLMEMLESPH 5Z12 Chain:C ((6-228)) ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ERILEAELAVEP---------------VTNICQAADKQLFTLVEWAKRIPHFSELPLDDQVILLRAGWNELLIASFSHRSIAVKDGILLATGLHV--------------HRNSAHSAGVGAIFDRVLTELVSKMRDMQMDKTELGCLRAIVLFNPDSKGLSNPAEVEALREKVYASLEAYCKHKYPEQPGRFAKLLLRLPALRSIGLKCLEHLFFFKLIGDTPIDTFLMEML----

General information: TITO was launched using: RESULT: Template: 5Z12.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 844 -114136 -135.23 -562.24 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain C : 0.76 3D Compatibility (PKB) : -135.23 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.262

(partial model without unconserved sides chains): PDB file : Tito_5Z12.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5z12-query.scw PDB file : Tito_Scwrl_5Z12.pdb: